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        胰酪大豆胨液體培養基TSB配方成分

        發布時間:

        2022-12-27

        作者:

        日水生物


        胰酪大豆胨液體培養基TSB胰酪大豆胨液體培養基[TSB]配制方法:稱取本品30.0g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],微溫溶解,分裝,121℃高壓滅菌15分鐘,備用。
         
        Trypticase Soy Broth Formula[配方]: g/L
         
        Pancreatic Digest of  Casein[胰酪胨]:17.0g
        Enzymatic Digest of Soybean  Meal[大豆木瓜蛋白酶水解物]:3.0g                                  
        Sodium Chloride[氯化鈉]:5.0g
        Dextrose•H2O [一水合葡萄糖]:2.5g
        Dipotassium Phosphate [磷酸氫二鉀]:2.5g
        pH7.3±0.2[25℃]
         
        The preparation method of TSB liquid medium: 30.0g of the product was weighed in 1L distilled water or deionized water [the amount of preparation can be increased or reduced in proportion], dissolved at micro-temperature, separated and sterilized at 121 C for 15 minutes.
        Trypticase Soy Broth Formula[formula]:g/L
        Pancreatic Digest of Casein[casein peptone]:17.0g
        Enzymatic Digest of Soybean Meal[]:3.0g hydrolysate of soybean papain]:3.0g
        Sodium Chloride[NaCl]:5.0g
        Dextrose H2O [glucose hydrate]:2.5g
        Dipotassium Phosphate [potassium hydrogen phosphate two potassium]:2.5g
        PH7.3 + 0.2[25 C]
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