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        改良胰蛋白胨大豆肉湯(mTSB)基礎制備方法

        發布時間:

        2022-12-27

        作者:

        山東培養基公司日水生物


        改良胰蛋白胨大豆肉湯(mTSB)基礎干粉培養配制方法:稱取本品33.0g于1L蒸餾水或去離子水
        中(可按比例增加或減少配制量),加熱溶解,分裝,121℃高壓滅菌15分鐘,冷至50-55℃每225ml培養基中添加1支21601,備用。資料來自青島日水生物培養基。
         
        配方: g/L
        胰酪蛋白胨:17.0g
        植物蛋白胨:3.0g
        D-葡萄糖:2.5g
        3號膽鹽:1.5g
        氯化鈉:5.0g
        磷酸氫二鉀:4.0g
        pH7.4±0.2(25℃)
        Modified tryptone soy broth (mTSB) basic dry powder culture and preparation method: the product is called 33.0g 1L distilled water or deionized water.
        In the medium (which can be increased or reduced proportionately), heat dissolving, packing, sterilizing at 121 degrees centigrade for 15 minutes, and adding 1 branches and 21601 pieces per 225ml medium to 50-55 centigrade.
        Formula: g/L
        Casein peptone: 17.0g
        Plant peptone: 3.0g
        D- glucose: 2.5G
        No. 3 bile salt: 1.5g
        Sodium chloride: 5.0g
        Two potassium hydrogen phosphate: 4.0g
        PH7.4 + 0.2 (25 C)
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